Cuadernos de Herpetología <p><strong>Cuadernos <em>de</em> Herpetología</strong> es una revista multi-disciplinaria que lidera en Argentina la publicación de trabajos científicos relacionados con Anfibios y Reptiles, abarcando tópicos como: sistemática, taxonomía, anatomía, fisiología, embriología, evolución, ecología, genética, comportamiento, conservación, paleontología, etc. Comprende las siguientes secciones: Trabajos, Revisiones, Notas y Novedades Zoogeográficas. Los idiomas aceptados son castellano, portugués e inglés.</p> <p><strong>Cuadernos <em>de</em> Herpetología</strong> se publica dos veces al año, Mayo y Septiembre.</p> <p><strong>Cuadernos <em>de</em> Herpetología</strong> no cobra a los autores por su publicación a excepción de manuscritos que contengan un número elevado de fotografías o gráficos estipulando un pago con los autores de acuerdo a los costos de edición de cada trabajo. Está disponible en formato electrónico como revista de Acceso Abierto (bajo una <a href="" rel="license">Licencia Creative Commons Atribución-NoComercial 4.0 Internacional</a>).</p> <p>Es una revista de la Asociación Herpetológica Argentina. ISSN en línea 1852-5768.</p> <p><img src="" alt="" width="381" height="132" /> <img src="" alt="Creative Commons License" /> </p> <p> </p> Asociación Civil Herpetológica Argentina es-ES Cuadernos de Herpetología 0326-551X <p><strong>Atribución-NoComercial 4.0 Internacional (CC BY-NC 4.0)</strong><br /><br />Usted es libre de:</p> <ul class="license-properties col-md-offset-2 col-md-8" dir="ltr"> <li class="license by"> <p><strong>Atribución</strong> — Usted debe dar <a id="appropriate_credit_popup" class="helpLink" tabindex="0" title="" href="" data-original-title="">crédito de manera adecuada</a>, brindar un enlace a la licencia, e <a id="indicate_changes_popup" class="helpLink" tabindex="0" title="" href="" data-original-title="">indicar si se han realizado cambios</a>. Puede hacerlo en cualquier forma razonable, pero no de forma tal que sugiera que usted o su uso tienen el apoyo de la licenciante.<span id="by-more-container"></span></p> </li> <li class="license nc"> <p><strong>NoComercial</strong> — Usted no puede hacer uso del material con <a id="commercial_purposes_popup" class="helpLink" tabindex="0" title="" href="" data-original-title="">propósitos comerciales</a>.</p> </li> <li class="license"><strong>No hay restricciones adicionales</strong> — No puede aplicar términos legales ni <a id="technological_measures_popup" class="helpLink" tabindex="0" title="" href="" data-original-title="">medidas tecnológicas que restrinjan legalmente a otras a hacer cualquier uso permitido por la licencia.</a></li> </ul> Dos nuevas especies del grupo Pristimantis boulengeri (Anura: Strabomantidae) de la cuenca alta del río Napo, Ecuador <p>Through the combination of morphological and phylogenetic evidence, we describe two species of Pristimantis from the upper basin of the Napo River. Both species have well-defined dorsolateral folds, a conical tubercle on the eyelid, a papilla on the tip of the snout, weakly expanded discs, and small size (female SVL &lt; 28.2 mm). Pristimantis omarrhynchus sp. nov. differs from<br>its sister species, Pristimantis miltongallardoi sp. nov., by the absence of iridophores on the belly, subacuminate snout in dorsal view, and narrow digits. Our phylogeny and morphological evidence, are conclusive in assigning them to the Pristimantis boulengeri species group, closely related to P. boulengeri, P. cryptopictus, P. dorspictus, and P. brevifrons. The new species are the first reported for the P. boulengeri group in Ecuador and the Amazon basin. We also comment on the correct identity of GenBank sequences previously assigned to P. thymelensis and P. myersi.</p> Patricia Bejarano-Muñoz Santiago R. Ron María José Navarrete Mario H. Yánez-Muñoz Derechos de autor 2022 Cuadernos de Herpetología 2022-09-29 2022-09-29 36 2 Parasitic helminths in Boana pulchella (Duméril & Bibron, 1841) (Anura: Hylidae) and their relation with host diet, body size, and habitat <p>We analyzed the diet and helminthological fauna of the frog Boana pulchella from the extreme south of Brazil. A total of 100 males were collected from two wetland areas in the state of Rio Grande do Sul: Ilha dos Marinheiros (n = 50) in the Rio Grande municipality, and the UFPel/ Embrapa (n = 50) in the Capão do Leão municipality. Boana pulchella food items and helminths<br>found in different organs were identified and quantified. We analyzed the relationship between helminth assemblage and host diet, body size, and sampling sites. Boana pulchella presented a generalist diet, composed mainly of terrestrial insects, with diet richness higher at Ilha dos Marinheiros. Helminth fauna was composed of Nematoda, Cestoda, Digenea, and Acanthocephala,<br>with no difference in helminth richness and abundance between sampling sites. However, the abundance of helminths presented a significant correlation with the volume of items found in the gastrointestinal contents of the anurans from UFPel/Embrapa-CL. Although for some helminth taxa there were significant differences in prevalence and mean intensity of infection among host size classes, the GLM (generalized linear model) between helminth abundance and anuran<br>SVL (snout-vent length) was not significant. Oxyascaris oxyascaris, Cosmocercinae gen. spp., Ochoterenella sp., Diplostomidae gen. spp., Pseudoacanthocephalus sp., and Centrorhynchus sp. were the main taxa constituting the helminth assemblage associated to B. pulchella males at the sampling sites. The occurrence of helminths at larval and adult stages suggests that B. pulchella may occupy different trophic levels in the biological cycles of those helminths. This helminth parasitic fauna associated with B. pulchella is mainly composed of taxa with heteroxenous cycles involving several intermediate and paratenic hosts, which agrees with the observations of a typical generalist anuran diet in this species.</p> Emily Costa Silveira Carolina Silveira Mascarenhas Sônia Huckembeck Gertrud Müller Daniel Loebmann Derechos de autor 2022 Cuadernos de Herpetología 2022-09-21 2022-09-21 36 2 DNA barcoding in Neotropical tadpoles: evaluation of 16S rRNA gene for the identification of anuran larvae from northeastern Brazil <p>The challenge in studying Neotropical tadpoles is identifying species using only their external morphology. However, the DNA barcode protocol is often implemented to help elucidate taxonomic issues. In fact, the identification of frogs through their unknown tadpoles has already been achieved accurately using this protocol. Despite the successful application of this tool, the efficiency of the 16S rRNA gene as a DNA barcode for Neotropical tadpoles has not been fully assessed. Herein we evaluate the efficacy of the 16S rRNA gene for identifying tadpoles from northeastern Brazil. Samples of 100 tadpole specimens from 12 locations were analyzed. The DNA sequences were individually submitted to a BLAST search and were then aligned with a matrix containing available sequences in the GenBank based on the anurans known to occur in the study area. The 16S rRNA fragment successfully identified the analyzed anuran species. Based on DNA barcoding, 8% of the tadpoles morphologically identified at the species level were incorrect. When an incongruence between morphological and molecular identifications was detected, the morphology of the target morphotype was reexamined, and previously neglected morphological characteristics were identified. DNA barcoding using the 16S rRNA gene facilitated the assessment of tadpole richness in northeastern Brazil. This DNA protocol can be used as a starting point for detecting high levels of genetic divergence, highlighting potential taxa that should be studied from phylogenetic and taxonomic perspectives.</p> Marcos J. Matias Dubeux Filipe A. Cavalcanti do Nascimento Larissa L. Correia Tamí Mott Derechos de autor 2022 Cuadernos de Herpetología 2022-08-19 2022-08-19 36 2 Herpeto-commerce: A look at the illegal online trade of amphibians and reptiles in Brazil <p>The illegal sale of fauna and flora represents the third-largest illegal trade in the world. Social media has contributed considerably to the increase in this type of trade. We searched for posts announcing the sale of amphibians and reptiles in seven Facebook® groups (three public and four private groups) from 01 January 2019 to 31 July 2020. In total, we found 548 posts made by a total of 201 social network profiles announcing the sale of 1,049 animals. We found 58 herpetofauna species being traded in the network (15 amphibian and 43 reptile species). Most of the sale advertisements originated in Southeast Brazil, predominantly from the state of São Paulo. The most traded species were Pantherophis guttatus (N= 467), Eublepharis macularius (N= 152), and Boa constrictor (N=90). This study presents important data about the illegal herpetofauna trade through Facebook® in Brazil, proving this market is currently fully active. This trade has high growth potential, bringing possible risks to biodiversity and public health. In conclusion, we recommend the implementation of urgent, specific government measures for its regulation and effective inspection.</p> Ibrahim Kamel Rodrigues Nehemy Thayllon Orzechowsky Gomes Fernanda Paiva Wesley Kauan Kubo João Emílio de Almeida Júnior Nathan Fernandes Neves Vinicius de Avelar São Pedro Derechos de autor 2022 Cuadernos de Herpetología 2022-09-06 2022-09-06 36 2 Exploring the morphological diversity of Patagonian clades of Phymaturus (Iguania: Liolaemidae). Integrative study and the description of two new species <p>In the present contribution, we revisited the taxonomy and phylogenetic relationships within the somuncurensis and spurcus clades of Phymaturus lizards. Based on 296 morphological characters and DNA sequences, we evaluated the taxonomic species status of each clade and of two populations sampled in a field trip. Based on this evidence, we describe two new taxa<br>for the genus. We also studied the species recently described for Chubut province, we analyzed their phylogenetic relationships, and compared them with other Patagonian species. Here, we provide data of color in life, squamation, and measurements, and compare in detail the new taxa to other members of their respective clades. We found that the somuncurensis clade comprises nine species (plus two other candidate ones) distributed mostly peripherally to the Somuncurá plateau, and present on the margins of it in isolated creeks and small mountain chains. Recent molecular studies arrived to different conclusions about the taxonomic validity of closely related species of the spurcus clade of Phymaturus lizards. We decided to revisit this group and contribute with a more complete analysis for two reasons: none of these studies revisited carefully the overall morphology and type series, and the only article that revisited this complex of species studied only one color pattern character, without providing voucher information (matching color types-collection specimens-DNA samples-sites). We studied the type series of all species, revisited characters taken from squamation and measurements, revised the color<br>pattern of all terminals, and performed statistical analysis. Our results discovered statistically significant characters, which provide enough morphological support to consider all species of the group as valid in congruence to the multilocus analysis that combined mitochondrial and nuclear data published recently. We also provide discrete color pattern characters that help to adequately differentiate these species.</p> Fernando Lobo Diego Andrés Barrasso Soledad Valdecantos Alejandro Giraudo Diego Omar Di Pietro Néstor G. Basso Derechos de autor 2022 Cuadernos de Herpetología 2022-09-29 2022-09-29 36 2 First record of myiasis in Physalaemus cuvieri Fitzinger, 1826 (Anura: Leptodactylidae) by Diptera <p>Myiasis is a parasitic disease caused by some species of Diptera, whose larvae feed on their host’s tissues. Records of myiasis in Brazilian anurans are scarce in the literature with single records in 12 species divided into 5 families. We report herein the observation of the first case of myiasis in the frog Physalaemus cuvieri from an individual found dead.</p> Bryan da Cunha Martins Leandro Silva Barbosa Rafael Scherrer Mathielo Derechos de autor 2022 Cuadernos de Herpetología 2022-08-08 2022-08-08 36 2 New additions to the anuran fauna of the Cancão Municipal Natural Park, Serra do Navio, state of Amapá, Brazil <p>Twelve species of anurans (Amazophrynella teko, Rhinella castaneotica, Hyalinobatrachium mondolfii, H. tricolor, Pristimantis gutturalis, P. inguinalis, Ranitomeya variabilis, Osteocephalus leprieurii, Scinax proboscideus, Leptodactylus petersii, Chiasmocleis hudsoni, Synapturanus mirandaribeiroi), into the seven families (Bufonidae, Centrolenidae, Craugastoridae, Dendrobatidae, Hylidae, Leptodactylidae, Microhylidae) are reported for the first time from Cancão Municipal Natural Park, state of Amapá, North Brazil. The total number of species of anurans known from the park now stands at 61 species. Our results contribute to an increase in the knowledge of the anuran fauna of Eastern Amazonia and Guiana Shield.</p> Carlos Eduardo Costa-Campos Patrick Ribeiro Sanches Fillipe Pedroso-Santos Vinicius A. M. B. de Figueiredo Rodrigo Tavares-Pinheiro Derechos de autor 2022 Cuadernos de Herpetología 2022-08-25 2022-08-25 36 2 Amelanism in Amphisbaena darwinii Duméril & Bibron, 1839 (Squamata: Amphisbaenidae) <p>Color anomalies are rarely reported in Amphisbaenia. We present the first record of amelanism in this group based on a specimen of Amphisbaena darwinii from Argentina. The photos were uploaded to a citizen science platform, reinforcing the positive impact of citizen science to filling gaps in our knowledge about biodiversity.</p> Carolina L. Paiva Mateo Cocimano Ricardo Montero Henrique C. Costa Derechos de autor 2022 Cuadernos de Herpetología 2022-08-25 2022-08-25 36 2 Hidden among bromeliads in the Brazilian semiarid: first records of Phyllopezus lutzae for the Caatinga domain and its predation by Tropidurus hispidus <p>During wildlife rescue and monitoring activities, we recorded 142 individuals of Phyllopezus lutzae in the municipalities of Tucano and Nova Soure, state of Bahia, Northeastern Brazil. These records are the first of this species in the Caatinga domain. Moreover, an adult individual of Tropidurus hispidus was recorded attempting to subdue an adult P. lutzae. Beyond to expand the known distribution range of the species, our records show that P. lutzae inhabits an ecological and climate domain different from Atlantic Forest where it was previously known, and that it is a potential prey of T. hispidus.</p> Alcéster Diego Coelho-Lima Oberdan Coutinho Nunes George Washington Neves Soares Tarcísio Jesus Santana Ericarla Barbosa Santana Alexandre Magno Pais Araújo Elaine Larissa Cardoso Lima Vashtir Ramalho dos Santos Braga Cristiano Eduardo Amaral Silveira-Júnior Arthur de Souza Magalhães Lyse Panelli de Castro Meira Maria José Pereira Fernandes Daniel Cunha Passos Derechos de autor 2022 Cuadernos de Herpetología 2022-08-24 2022-08-24 36 2 Novos registros dos lagartos Ameivula cipoensis Arias et al., 2014, Enyalius capetinga Breitman et al., 2018, Psilops paeminosus (Rodrigues, 1991) e Tupinambis quadrilineatus Manzani & Abe, 1997 (Squamata) para o estado de Minas Gerais, Brasil, através da Henrique J. Oliveira Henrique C. Costa Derechos de autor 2022 Cuadernos de Herpetología 2022-09-06 2022-09-06 36 2